PhosphoSens Kinetic Assays Deliver Rich Datasets in Multiple Formats With One Technology

AssayQuant's kinetic assays are continuous by design enabling direct detection and quantification of kinase activity. Each assay is optimized using our proprietary CSox fluorophore covalently bound to a physiologically derived peptide substrate. PhosphoSens^® Technology is, at the same time, novel and non-disruptive – delivering a progress curve in every well using a simple add-and-read workflow and standard fluorescence microplate reader. PhosphoSens kinetic assays yield rich datasets, making them an ideal successor to platforms like mobility shift and radioactive assays.

A simple workflow to accelerate your drug discovery and research capabilities. Robust and flexible to fit your scientific needs and research continuum.

Thanks to superior peptide substrates and a small, minimally hydrophobic fluorophore design, the PhosphoSens platform is ideal for today's research. The simple add-and-read workflow produces highly quantitative data for three application areas: PhosphoSens-Kinetic, PhosphoSens-Red and PhosphoSens-Lysate for kinetic, end-point and crude cell/tissue lysate, respectively.

In the kinetic format, the actual catalytic event of phosphorylation generates the fluorescence signal which is measured in real-time capturing the complete progress curve for every condition with no additional handling. For the end-point format, simply add Europium to the kinetic assay at any point during the progress curve to yield a red-shifted, time-resolved assay to enable correction for compound auto-fluorescence and batch mode processing for high throughput applications.

PhosphoSens-Kinetic is ideal for Profiling, Potency & MOI/MOA. PhosphoSens-Lysate works the same as kinetic but is designed for crude cell or tissue lysates. 

PhosphoSens-Red (End-Point) is ideal for high volumes of tests for HTS.